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Molecular Biology
In Vitro Transcription / Translation
In Vitro Transcription Kits
HiScribe T7 High Yield RNA Synthesis Kit
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HiScribe T7 High Yield RNA Synthesis Kit
HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs
(2)
Write a review
Citations:
(283)
Supplier Page
Supplier Page from
New England Biolabs for
HiScribe T7 High Yield RNA Synthesis Kit
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
HiScribe T7 High Yield RNA Synthesis Kit
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
E2040S
Quantity
50 reactions
Applications
Functional Genomics
Brand/Trademark
New England Biolabs Inc (NEB)
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (283)
(1)
Programmable modulation of ribosomal frameshifting by mRNA targeting CRISPR-Cas12a system
iScience
December 15, 2023
Shih-Hong Huang, Shih-Cheng Chen, Tsu-Ying Wu, Cheng-Yao Chen, Chien-Hung Yu
DNA Assembly
Cloning
Kit,
New England Biolabs
,Cat#
E5520S
T7 RNA Polymerase
,
New England Biolabs
,Cat#
E2040S
SP6 RNA Polymerase
,
New England Biolabs
,Cat#
E2070S
Anti-Reverse Cap Analog (ARCA),
New England Biolabs
,Cat#
(2)
Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos
Molecular Therapy - Nucleic Acids
December 12, 2023
Weikai Ge, Xiaozhu Zhao, Shixue Gou, Qin Jin, Fangbing Chen, Zhen Ouyang, Chengdan Lai, Tao Cui, Baoyi Mai, Sijia Lu,
et al.
using the
HiScribe T7 ARCA mRNA Kit
(
NEB
,
E2060S
) and
HiScribe T7 High Yield RNA Synthesis Kit
(
NEB
,
E2040S
) and harvested RNA using the RNeasy MinElute Cleanup Kit (
Qiagen
,
12243
). The final concentrations of
(3)
The ribosome‐associated chaperone Zuo1 controls translation upon TORC1 inhibition
The EMBO Journal
December 11, 2023
Ailsa Black, Thomas D Williams, Flavie Soubigou, Ifeoluwapo M Joshua, Houjiang Zhou, Frederic Lamoliatte, Adrien Rousseau
Coprecipitant,
Thermo Fisher
,Cat #
AM9515
HiScribe T7 High Yield RNA synthesis Kit
,
New England Biolabs
,Cat#
E2040S
Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
RNeasy Mini Kit,
Qiagen
,Cat
(4)
Purification and functional validation of LtCas12a protein
STAR Protocols
December 1, 2023
Bo Zhou, Ye Chen, Lifang Li, Jiashuo Liu, Yuyan Wang, Zheying Huang, Zheng Hu, Rui Tian, Zhen Li
assays
,, SDS-PAGE Gel Preparation Kit,Yeasen,Cat#20328ES50
HiScribe T7 High Yield RNA Synthesis Kit
,
NEB
,Cat#
E2040S
Monarch
RNA Cleanup
Kit,
NEB
,Cat#
T2040L
EasyPure® Quick Gel Extraction Kit,Trans,Cat#EG101-01 Lateral
(5)
Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes
Nature Communications
November 14, 2023
Ren Ren, Shenglin Cai, Xiaona Fang, Xiaoyi Wang, Zheng Zhang, Micol Damiani, Charlotte Hudlerova, Annachiara Rosa, Joshua Hope, Nicola J. Cook,
et al.
amplicons were transcribed to single-stranded RNA using HiScribeTM T7 High Yield RNA Synthesis Kit (
E2040S
,
New England Biolabs
). 5 µl of DNA amplicon products were mixed with 1.5 µl of T7 reaction buffer (10×),
(6)
Proenkephalin-A secreted by renal proximal tubules functions as a brake in kidney regeneration
Nature Communications
November 7, 2023
Chi Liu, Xiaoliang Liu, Zhongwei He, Jiangping Zhang, Xiaoqin Tan, Wenmin Yang, Yunfeng Zhang, Ting Yu, Shuyi Liao, Lu Dai,
et al.
PCR amplification
. Subsequently, the gRNA was synthesized using a
Hiscribe T7 High Yield RNA Synthesis Kit
(
NEB
,
E2040S
). For
CRISPR/Cas9
mutagenesis
, a mixed solution containing 1 nL of Cas9 protein (0.7 μM,
NEB
,
M0646T
)
(7)
Genome-wide analysis of the interplay between chromatin-associated RNA and 3D genome organization in human cells
Nature Communications
October 16, 2023
Riccardo Calandrelli, Xingzhao Wen, John Lalith Charles Richard, Zhifei Luo, Tri C. Nguyen, Chien-Ju Chen, Zhijie Qi, Shuanghong Xue, Weizhong Chen, Zhangming Yan,
et al.
in vitro transcription
by
T7 RNA polymerase
, using the
HiScribe T7 High Yield RNA Synthesis kit
(
New England Biolabs
-
E2040S
) following the manufacturer’s instructions. Resulting transcription reactions were treated with DNAse
(8)
Massively parallel identification of sequence motifs triggering ribosome-associated mRNA quality control
bioRxiv
October 4, 2023
Katharine Y. Chen, Heungwon Park, Arvind Rasi Subramaniam
cassette, similar to previous approaches27,86, using the
HiScribe T7 High Yield RNA Synthesis Kit
(
NEB
E2040S
). Transcribed gRNA was cleaned using the RNA Clean and Concentrator kit (Zymo R1013). Library mRNA
(9)
Click display: a rapid and efficient in vitro protein display method for directed evolution
Nucleic Acids Research
September 8, 2023
Yu Zeng, Michael Woolley, Karuppiah Chockalingam, Benjamin Thomas, Srishtee Arora, Magnus Hook, Zhilei Chen
synthesis (1 μg per 20 μl reaction,
HiScribe™ T7 High-Yield RNA Synthesis Kit
(
New England Biolabs
,
E2040S
)). At the end of the reaction, 70 μl nuclease-free water, 10 μl 10× DNase I Buffer, and 2 μl DNase I
(10)
Programmable RNA detection with CRISPR-Cas12a
Nature Communications
September 5, 2023
Santosh R. Rananaware, Emma K. Vesco, Grace M. Shoemaker, Swapnil S. Anekar, Luke Samuel W. Sandoval, Katelyn S. Meister, Nicolas C. Macaluso, Long T. Nguyen, Piyush K. Jain
amplified product was
in-vitro
transcribed using the
HiScribe T7 High Yield RNA synthesis kit
(
NEB
#
E2040S
) following the manufacturer’s protocol. The transcribed product was treated with DNase I for 30 min
(11)
An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments
STAR Protocols
September 1, 2023
Xiaoqian Zeng, Shuliu Wang, Mindong Liang, Weishan Wang, Yue Jiang, Fei Xu, Leshi Liu, Hao Yan, Yaojun Tong, Lixin Zhang,
et al.
generate crRNA pairs (Figure 2). NEB HiScribeTM T7 Quick High Yield
RNA Synthesis
Kit (
NEB
, Catalog#
E2040S
) is used in this step.a.Prepare 100 μL
in vitro transcription
master mix (see materials and equipment)
(12)
Single-cell massively-parallel multiplexed microbial sequencing (M3-seq) identifies rare bacterial populations and profiles phage infection
Nature Microbiology
August 31, 2023
Bruce Wang, Aaron E. Lin, Jiayi Yuan, Katherine E. Novak, Matthias D. Koch, Ned S. Wingreen, Britt Adamson, Zemer Gitai
SPRI and used for
in vitro transcription
. Guide RNAs were transcribed using the NEB HiScribe kit (
NEB
E2040S
) by mixing 100 ng of DNA template, 2 μl of 10× reaction buffer, 2 μl 100 mM ATP, 2 μl 100 mM GTP, 2
(13)
The non-redundant functions of PIWI family proteins in gametogenesis in golden hamsters
Nature Communications
August 29, 2023
Xiaolong Lv, Wen Xiao, Yana Lai, Zhaozhen Zhang, Hongdao Zhang, Chen Qiu, Li Hou, Qin Chen, Duanduan Wang, Yun Gao,
et al.
and amplify sgRNAs
in vitro transcription
using the
HiScribe T7 High Yield RNA Synthesis Kit
(
NEB
,
E2040S
). The complementary target oligonucleotides were cloned into the pUC57-T7-sgRNA-trcRNA vector through
(14)
In vitro Analysis of Stalled Ribosomes Using Puromycin Incorporation
Bio Protoc
August 20, 2023
MaKenzie R. Scarpitti, Michael G. Kearse
catalog number: 4401457)
HiScribe T7 High Yield RNA Synthesis kit
(
New England Biolabs
, catalog number:
E2040S
)iScript Reverse Transcription Supermix (
Bio-Rad
, catalog number:
1708841
)
iTaq Universal SYBR Green Supermix
,
(15)
Transcriptional and chromatin profiling of human blood innate lymphoid cell subsets sheds light on HIV‐1 pathogenesis
The EMBO Journal
August 15, 2023
Yetao Wang, Lawrence Lifshitz, Noah J Silverstein, Esther Mintzer, Kevin Luk, Pamela StLouis, Michael A Brehm, Scot A Wolfe, Steven G Deeks, Jeremy Luban
and subjected to
in vitro transcription
(IVT) using
HiScribe T7 High Yield RNA Synthesis Kit
(
NEB
,
E2040S
), then treated with ExoSAP‐IT (Affymetrix, 78200). IVT RNA was fragmented using
RNA fragmentation reagents
(16)
Multiple TMA-aided CRISPR/Cas13a platform for highly sensitive detection of IL-15 to predict immunotherapeutic response in nasopharyngeal carcinoma
J Immunother Cancer
August 3, 2023
Ya-Xian Wu, Shan Xing, Yu Wang, Bo-Yu Tian, Meng Wu, Xue-Ping Wang, Qi Huang, Xia He, Shu-Lin Chen, Xiao-Hui Li,
et al.
RE0082; RE0083). The
HiScribe T7 High Yield RNA Synthesis Kit
was purchased from
New England Biolabs
(
E2040S
). The v2 reporter was purchased from
Thermo Scientific
(Massachusetts, USA;
4479769
). Streptavidin-coated
(17)
IFI16 phase separation via multi-phosphorylation drives innate immune signaling
Nucleic Acids Research
July 21, 2023
Dawei Liu, Krystal K Lum, Nicholas Treen, Corazón T Núñez, Jinhang Yang, Timothy R Howard, Michael Levine, Ileana M Cristea
Alexa Fluor™ 488 Protein Labeling Kit
,
Thermo Fisher Scientific
,
A10235
HiScribe® T7 High Yield RNA Synthesis Kit
,
New England BioLabs
,
E2040S
Applied Biosystems
PowerUp SYBR Green Master Mix
,
Thermo Fisher Scientific
,
A25778
Oligonucleotides,Oligonucleotides,Oligonucleotides Cy5-VACV70mer
(18)
Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency
Nucleic Acids Research
July 21, 2023
Karthikeyan Ponnienselvan, Pengpeng Liu, Thomas Nyalile, Sarah Oikemus, Stacy A Maitland, Nathan D Lawson, Jeremy Luban, Scot A Wolfe
transcribed from 500 ng purified linearized template using the
HiScribe T7 High-Yield RNA Synthesis Kit
(
NEB
,
E2040S
) with co-transcriptional capping by CleanCap AG (TriLink Biotechnologies, N-7413-5) and full replacement
(19)
Human gain-of-function variants in HNF1A confer protection from diabetes but independently increase hepatic secretion of atherogenic lipoproteins
Cell Genomics
July 12, 2023
Natalie DeForest, Babu Kavitha, Siqi Hu, Roi Isaac, Lynne Krohn, Minxian Wang, Xiaomi Du, Camila De Arruda Saldanha, Jenny Gylys, Edoardo Merli,
et al.
were phenol/chloroform-extracted and
in vitro
-transcribed with
T7 RNA Polymerase
HiScribe Mix (
NEB
E2040S
), CleanCap Reagent AG (Tri-Link N-7113) and 5-methoxyuridine triphosphate instead of UTP for 2 h at
(20)
RAD21 is the core subunit of the cohesin complex involved in directing genome organization
Genome Biology
June 28, 2023
Yuao Sun, Xin Xu, Wenxue Zhao, Yu Zhang, Keyang Chen, Yongzheng Li, Xiaotian Wang, Mengling Zhang, Boxin Xue, Wanting Yu,
et al.
then converted into RNA via
in vitro transcription
(
HiScribe™ T7 High Yield RNA Synthesis Kit
NEB
, #
E2040S
). RNA templates were converted back to DNA via reverse transcription (MAN0012047 TS Maxima H Minus Reverse
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Reviews
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(1)
7/23/2023
RNA Synthesis Kit From NEB
Ganesh Pusapati
The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying...
Read Review
(1)
4/26/2022
Great RNA Synthesis Kit
Nan Wang
This is high fidelity RNA synthesis kit from New England Biolabs. The synthesized RNA can be used for in vivo microinjection to achieve the RNAi effect.
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HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs
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